Skripsi

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER

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Penilaian

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The objective of the research was to evaluate and characterisize of a Lactic Acid Bacteria isolated from broiler’s small intestine. The research was done by three steps : (1) Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria. It was conducted from March 26 to April 27, 2007 at Microbiology Laboratory of Agriculture Technology Department, Faculty of Agriculture, Sriwijaya University, Indralaya. The isolation of lactic acid bacteria was proceed by clone ffom broiler’s small intestine with MRS Broth for 24 hours, thinned until 10‘9 with serial number, invested in MRS jeli, purify by streak plate method and storaged the lactic acid bacteria in an MRS angle jeli. Gram colourize was done with crystal violet liquid, Iodium, Alcohol 95% and Safranin, and also miroscopic observation was done by electrone microscope with 40 zooming. Inhibitory test with pathogen bacteria was evaluated with Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 and 48 hours incubation period. The isolation result showed two sample of isolate (Al and A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate Al the colonies had perfect circle shape and white colour. The isolate of A2 had circle shape with mosaic and white broken colour. After that, gramcolouring was done and microscopic observation were identified into gram- negatife bacteria became bacill and bacill cocco. Inhibitory test with pathogen bacteria showed that it produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactbacillus sp as the genera of Laclid Acid Bacteria.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
0707000773	T72794	T727942007	Central Library (REFERENCES)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T727942007

Publisher

Inderalaya : Prodi Nutrisi dan Makanan Ternak, Fakultas Pertanian Universitas Sriwijaya., 2007

Collation

xvii, 27 hlm.; ill.; tab.; 28 cm.

Language

Indonesia

ISBN/ISSN

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Classification

636.5

Content Type

Text

Media Type

unmediated

Carrier Type

unspecified

Edition

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Subject(s)

Prodi Nutrisi dan Makanan Ternak
Peternakan ayam

Specific Detail Info

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Statement of Responsibility

Furika

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