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Image of ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER

Skripsi

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER

Merint, Merint - Personal Name;

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Penilaian anda saat ini :  

The objective of the research was to evaluate and characterisize of a Lactic Acid Bacteria isolated from broiler’s small intestine. The research was done by three steps : (1) Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria. It was conducted from March 26 to April 27, 2007 at Microbiology Laboratory of Agriculture Technology Department, Faculty of Agriculture, Sriwijaya University, Indralaya. The isolation of lactic acid bacteria was proceed by clone ffom broiler’s small intestine with MRS Broth for 24 hours, thinned until 10‘9 with serial number, invested in MRS jeli, purify by streak plate method and storaged the lactic acid bacteria in an MRS angle jeli. Gram colourize was done with crystal violet liquid, Iodium, Alcohol 95% and Safranin, and also miroscopic observation was done by electrone microscope with 40 zooming. Inhibitory test with pathogen bacteria was evaluated with Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 and 48 hours incubation period. The isolation result showed two sample of isolate (Al and A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate Al the colonies had perfect circle shape and white colour. The isolate of A2 had circle shape with mosaic and white broken colour. After that, gramcolouring was done and microscopic observation were identified into gram- negatife bacteria became bacill and bacill cocco. Inhibitory test with pathogen bacteria showed that it produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactbacillus sp as the genera of Laclid Acid Bacteria.


Availability
Inventory Code Barcode Call Number Location Status
0707000773T72794T727942007Central Library (REFERENCES)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T727942007
Publisher
Inderalaya : Prodi Nutrisi dan Makanan Ternak, Fakultas Pertanian Universitas Sriwijaya., 2007
Collation
xvii, 27 hlm.; ill.; tab.; 28 cm.
Language
Indonesia
ISBN/ISSN
-
Classification
636.5
Content Type
Text
Media Type
unmediated
Carrier Type
unspecified
Edition
-
Subject(s)
Prodi Nutrisi dan Makanan Ternak
Peternakan ayam
Specific Detail Info
-
Statement of Responsibility
Furika
Other version/related

No other version available

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  • ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER
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