Skripsi

EKSPRESI PROTEIN REKOMBINAN PEPTIDE YY (PYY) PADA Escherichia coli

---

Penilaian

---

Background: PYY (1-36), a polypeptide consisting of 36 amino acid structures, is known to play a role in increasing the growth and survival of beta cells through NPY1R activation in the pancreas, which ultimately leads to glycemic balance. (P3L31P34)PYY(1-36) is a PYY (1-36) analog that is resistant to DPP-IV degradation and has high selectivity to NPY1R. This study aims to identify the characteristics of the (P3L31P34)PYY(1-36) recombinant protein-coding gene and its prediction of homologous proteins. Methods: This study is an experimental study with a computational approach. Data in the form of amino acid sequences were accessed from Pubmed and the iGEM registry. This study uses several website-based applications including OptimumGeneTM, Predict Protein, Protein-SOL, and SWISS-MODEL. Results: Construction 1 was characterized by a secondary structural composition consisting of α-helix and loop, and a solubility value of 0.5. While construction 2, the secondary structure composition contains α-helix, β-strand, and loop with a solubility value of 0.821. In the (P3L31P34)PYY(1-36) modelling, three homologous protein models were obtained. Conclusion: Construction 1 and 2 have different secondary structures. Meanwhile, the solubility value of construction 2 has a higher value. (P3L31P34)PYY(1-36) has three homologous protein models. Keywords: PYY (1-36), (P3L31P34)PYY(1-36), recombinant protein, computational approach

---

Availability

Inventory Code	Barcode	Call Number	Location	Status
2107000594	T40634	T406342021	Central Library (REFERENCES)	Available but not for loan - Not for Loan

Detail Information

Series Title

-

Call Number

T406342021

Publisher

Palembang : Prodi Kedokteran, Fakultas Kedokteran Universitas Sriwijaya., 2021

Collation

xv, 56 hlm.; tab.; ilus.; 29 cm.

Language

Indonesia

ISBN/ISSN

-

Classification

574.483 07

Content Type

Text

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Kedokteran Umum
Protein Rekombinan Peptide

Specific Detail Info

-

Statement of Responsibility

MI

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment