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Image of EXPLORATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM THE RHIZOSPHERE OF GELAM PLANTS (Melaleuca cajuputi Maton & Sm. Ex R. Powell) IN PEAT SOIL ARBORETUM OF SRIWIJAYA UNIVERSITY

Skripsi

EXPLORATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM THE RHIZOSPHERE OF GELAM PLANTS (Melaleuca cajuputi Maton & Sm. Ex R. Powell) IN PEAT SOIL ARBORETUM OF SRIWIJAYA UNIVERSITY

Insani, Firda Aulia - Personal Name;

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Peat Soil often present significant challenges for plant growth, primarily due to low pH and limited nutrient availability, especially phosphorus. Phosphorus is an essential macronutrient for plants, yet in peatlands, most of it exists in insoluble forms and cannot be directly utilized. This condition necessitates sustainable approaches to enhance phosphorus availability. Phosphate solubilizing bacteria (PSB) offer a promising natural solution, capable of converting insoluble phosphates into plant-available forms through mechanisms like organic acid production and phosphatase enzymes. This research, driven by an understanding of this problem and the potential of PSBs, aimed to Isolat PSBs from the rhizosphere of Melaleuca cajuputi in the peatlands of the Sriwijaya University Arboretum, evaluate their phosphate solubilizing ability qualitatively and quantitatively, and characterize potential Isolats. The exploration of PSBs from Melaleuca cajuputi is relevant as these plants have adapted well to the extreme conditions of peatlands, implying an association with similarly adaptive microorganisms. A total of 8 bacterial isolates were successfully obtained from the Melaleuca cajuputi rhizosphere. Qualitative screening confirmed that all isolates possessed phosphate-solubilizing ability, indicated by the formation of clear zones on Pikovskaya agar medium. Furthermore, quantitative tests showed variations in phosphate solubilization capabilities, with isolate PG2 demonstrating the highest ability on day 2 (57.361 ppm) and day 4 (49.072 ppm) of incubation. Media pH measurements showed a decrease in pH correlating with phosphate solubilization ability, indicating that the mechanism of phosphate solubilization by these bacteria likely involves organic acid production, which was also supported by organic acid production tests. Consequently, this study concludes that the phosphate-solubilizing bacteria isolated from the Melaleuca cajuputi rhizosphere have great potential for development as biofertilizers agents to enhance phosphorus availability in peatlands and sustainably support plant growth. Keyword: Phosphate-solubilizing bacteria, Melaleuca cajuputi, peatland, rhizosphere. Tanah gambut seringkali menghadapi tantangan besar bagi pertumbuhan tanaman, terutama disebabkan oleh pH rendah dan ketersediaan nutrisi yang terbatas, khususnya fosfor. Fosfor merupakan unsur hara makro esensial yang diperlukan tanaman, namun di lahan gambut, sebagian besar berada dalam bentuk tidak larut dan tidak dapat langsung dimanfaatkan. Kondisi ini menuntut pendekatan berkelanjutan untuk meningkatkan ketersediaan fosfor. Bakteri pelarut fosfat (BPF) menawarkan solusi alami yang menjanjikan, mampu mengubah fosfat tidak larut menjadi bentuk yang dapat diserap tanaman melalui produksi asam organik dan enzim fosfatase. Penelitian ini didasari oleh pemahaman akan masalah ini dan potensi BPF, dengan tujuan mengisolasi BPF dari rizosfer Melaleuca cajuputi di lahan gambut Arboretum Universitas Sriwijaya, mengevaluasi kemampuan isolat dalam melarutkan fosfat secara kualitatif dan kuantitatif, serta mengkarakterisasi isolat potensial. Eksplorasi BPF dari Melaleuca cajuputi relevan karena tumbuhan ini telah beradaptasi dengan baik pada kondisi lahan gambut yang ekstrem, menyiratkan asosiasi dengan mikroorganisme adaptif serupa. Dari penelitian ini, berhasil diisolasi sebanyak 8 isolat bakteri dari rizosfer Melaleuca cajuputi. Hasil skrining kualitatif menunjukkan bahwa semua isolat tersebut memiliki kemampuan melarutkan fosfat, yang ditandai dengan pembentukan zona bening pada media Pikovskaya agar. Selanjutnya, uji kuantitatif menunjukkan variasi kemampuan pelarutan fosfat, dengan isolat PG2 menunjukkan kemampuan tertinggi pada inkubasi hari ke-2 (57.361 ppm) dan hari ke-4 (49.072 ppm).Pengukuran pH media inkubasi menunjukkan penurunan pH seiring dengan kemampuan pelarutan fosfat, mengindikasikan bahwa mekanisme pelarutan fosfat oleh bakteri-bakteri ini kemungkinan besar melibatkan produksi asam organik, yang juga didukung oleh uji kemampuan produksi asam organik yang dilakukan. Dengan demikian, penelitian ini menyimpulkan bahwa bakteri pelarut fosfat yang diisolasi dari rizosfer Melaleuca cajuputi ini berpotensi besar untuk dikembangkan sebagai biofertilizer untuk meningkatkan ketersediaan fosfor di lahan gambut dan mendukung pertumbuhan tanaman secara berkelanjutan. Keyword: Bakteri pelarut fosfat, Melaleuca cajuputi, lahan gambut, rhizosfer.


Availability
Inventory Code Barcode Call Number Location Status
2507006267T185549T1855492025Central Library (Reference)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T1855492025
Publisher
Indralaya : Prodi Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2025
Collation
xviii, 99 hlm.; ilus.; tab.; 29 cm.
Language
Indonesia
ISBN/ISSN
-
Classification
579.307
Content Type
Text
Media Type
unmediated
Carrier Type
other (computer)
Edition
-
Subject(s)
Prodi Ilmu Biologi
Bakteri Pelarut
Specific Detail Info
-
Statement of Responsibility
MI
Other version/related
TitleEditionLanguage
EKPLORASI BAKTERI PELARUT FOSFAT DARI RHIZOSFER MANGROV DI KAWASAN TAMAN NASIONAL SEMBILANG SUMATERA SELATANid
ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT PADA TANAH ULTISOL DARI BEBERAPA WILAYAH DI SUMATERA SELATANid
UJI POTENSI DAN SINERGISME ISOLAT BAKTERI PENAMBAT NITROGEN DAN BAKTERI PELARUT FOSFAT DARI TANAH ULTISOL DI BEBERAPA WILAYAH DI PROVINSI SUMATERA SELATANid
File Attachment
  • EXPLORATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM THE RHIZOSPHERE OF GELAM PLANTS (Melaleuca cajuputi Maton & Sm. Ex R. Powell) IN PEAT SOIL ARBORETUM OF SRIWIJAYA UNIVERSITY
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