Skripsi

KOMPOSISI ALEL SPESIFIK PADA POPULASI IKAN NILA (Oreochromis niloticus) DI DANAU RANAU BERDASARKAN SNP MARKER

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Penilaian

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Nile tilapia (Oreochromis niloticus) is one of the important freshwater fish commodities in Indonesia that has various strains resulting from genetic selection. This study aimed to determine the morphological characteristics and species or strain composition of Nile tilapia that are cultivated or caught in Ranau Lake by utilizing Single Nucleotide Polymorphism (SNP) molecular markers through the KASP assay PCR method. The study was conducted from December 2024 to January 2025 in Ranau Lake and the Biomolecular Laboratory of Universitas Sriwijaya. The samples were consisted of 10 samples of culture Nile tilapia (DRB) and 10 samples of capture Nile tilapia (DRA). The research stages included sampling, DNA extraction, PCR amplification, DNA visualization, and detection of species-specific SNP. The results showed that the Nile tilapia population in Ranau Lake has morphological and genetic diversity, both in culture and capture. SNP marker analysis identified the presence of more than one strain or species of tilapia and revealed strong indications of hybridization, as seen from the mixed genotype pattern and overlapping morphometric and meristic characters between groups. The diagnostic allele frequency of capture Nile tilapia was 0.35 and its alternative allele was 0.65. The diagnostic allele frequency of culture Nile tilapia was 0.65 and the alternative allele was 0.35. Water quality in capture (DRA) indicated the value of pH 5.3-7.2, temperature 31.1-32.9°C, TDS 0.21-0.39 mg L-¹, dissolved oxygen 2.0-5.4 mg L-¹, transparency 45 cm, ammonia 0.052-0.156 mg L-¹ and alkalinity 158-226 mg L-¹. Meanwhile, water quality in the culture (DRB) denoted the value of pH 6.6-6.9, temperature 27.4-27.7 °C, TDS 0.13 mg L-¹, dissolved oxygen 5.3-5.4 mg L-¹, transparency 65 cm, ammonia 0.058-0.137 mg L-¹ and alkalinity 72.88 mg L-¹.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507006096	T185077	T1850772025	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1850772025

Publisher

Indralaya : Prodi Budidaya Perairan, Jurusan Perikanan Fakultas Pertanian Universitas Sriwijaya., 2025

Collation

xv, 67 hlm.; ilus.; tab, 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

639.310 7

Content Type

Text

Media Type

unmediated

Carrier Type

other (computer)

Edition

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Subject(s)

Prodi Budidaya Perairan
Peternakan Ikan Nila

Specific Detail Info

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Statement of Responsibility

MI

Other version/related

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