Skripsi

ISOLASI SENYAWA SESKUITERPEN LAKTON DARI FRAKSI ETIL ASETAT DAUN Tithonia diversifolia SERTA SKRINING SITOTOKSIK DENGAN METODE BSLT (Brine Shrimp Lethality Test)

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Penilaian

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Tithonia diversifolia is a shrub belonging to the Asteraceae family and one of 12 species classified under Tithonia genus. T. diversifolia is known in Indonesia as kipait, paitan, kembang bulan, and insulin. Previous studies have reported that T. diversifolia contains abundant sesquiterpene lactones compounds. Sesquiterpene lactones have been extensively reported in the aerial parts of T. diversifolia, without separating between stem and leaf parts. This study aims to isolate secondary metabolites from the ethyl acetate fraction of T. diversifolia leaves, followed by cytotoxicity testing. The isolation process involves extraction, fractionation, separation, and purification steps. Extraction was performed by maceration for 1 x 24 hours with methanol as the solvent, followed by liquid-liquid fractionation using n-hexane and ethyl acetate as solvents. Separation and purification of the compounds were carried out using various chromatographic techniques, including vacuum liquid chromatography, radial chromatography, and Sephadex column chromatography. The structure of the isolated compound was determined using UV spectroscopy and NMR (1H-NMR, 13C-NMR, HSQC, and HMBC). The isolated compound yielded 75.1 mg of a yellow solid. Structural elucidation revealed that the isolated compound belongs to the sesquiterpene lactone group with a germacrene skeleton named tagitinin C. Cytotoxicity testing using the BSLT method with Artemia salina larvae at test solution concentrations ranging from 100 to 1.56 mg/L indicated that the isolated compound was cytotoxic with an LC50 value of 37.26 ± 3.03 µg/mL. Keywords: Tithonia diversifolia, Sesquiterpene lactones, Brine Shrimp Lethality Test

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507004573	T180233	T1802332025	Central Library (REFERENCE)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1802332025

Publisher

Indralaya : Prodi Ilmu Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2025

Collation

xxii, 549 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

540.07

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

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Subject(s)

Prodi Ilmu Kimia
Seskuiterpen

Specific Detail Info

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Statement of Responsibility

TUTI

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