Skripsi

ISOLASI DAN KARAKTERISASI ENZIM α -GLUKOSIDASE DARI BERAS KETAN PUTIH (Oryza sativa L. var. glutinosa)

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Penilaian

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This study aims to determine the activity of the α-glucosidase enzyme from white glutinous rice (Oryza sativa L. var. glutinosa) and determine the optimum conditions of the enzyme at incubation time, temperature, pH and substrate concentration. The test of the activity of the α-glucosidase enzyme used the substrate p-Nitrophenyl-α-D-Glucopyranoside (pNPG). The kinetic parameters of the α-glucosidase enzyme were determined to obtain the Vmax and KM values based on the Lineweaver-Burk equation. The process of isolating the α glucosidase enzyme from white glutinous rice was carried out starting from the extraction of white glutinous rice flour using buffer, then fractionated using ammonium sulfate salt. The results of the study obtained a crude extract of the α-glucosidase enzyme from white glutinous rice having a specific enzyme activity of 0.023 U/mg with an enzyme activity of 0.039 U/mL and a protein content of 1.665 mg/mL. The results of ammonium sulfate fractionation had the highest specific activity in fraction III (40-60%) of 0.136 U/mg with enzyme activity of 0.048 U/mL and protein content of 0.353 mg/mL. The optimum conditions of the α-glucosidase enzyme from white glutinous rice showed optimum activity at an incubation time of 25 minutes, pH 5.8, temperature 37°C and substrate concentration of 0.015 M with enzyme activity of 0.123 U/mL. The kinetic parameter test of the α-glucosidase enzyme from white glutinous rice based on the Lineweaver-Burk equation had a Vmax value of 0.19 M/min and a KM value of 0.00987 M.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507004491	T180268	T1802682025	Central Library (REFERENCE)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1802682025

Publisher

Indralaya : Prodi Ilmu Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2025

Collation

xvi, 175 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

540.07

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Kimia

Specific Detail Info

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Statement of Responsibility

TUTI

Other version/related

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