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PENGARUH RESTRIKSI KALORI DAN OLAHRAGA TERHADAP EKSPRESI CASPASE-3 PADA HATI TIKUS YANG MENGONSUMSI ALKOHOL

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Penilaian

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Global alcohol consumption is expected to rise by 17% over the next 13 years, which could increase the risk of liver disease due to oxidative stress, inflammation, and the activation of caspase-3, a key protein of apoptosis. While caloric restriction (CR) and exercise are known to reduce oxidative stress and caspase-3 levels, research on their effects on liver cell apoptosis during alcohol exposure is limited. This study utilized a post-test-only controlled group design with 30 male Wistar rats, of which 25 met the inclusion criteria for analysis. The groups included a control, an alcohol group, and groups receiving alcohol along with either CR, exercise, or both. Alcohol was administered at 2.5 mL of 20% ethanol, CR was set at 20% of the standard diet, and exercise involved using a hurdle running wheel at 10-12 m/min for 30 minutes each session. After six weeks, caspase-3 levels were assessed through immunohistochemical analysis and quantified using ImageJ, followed by statistical evaluation. Results showed a significant increase in caspase-3 expression in the alcohol group compared to the control (p < 0.001), with averages of 14.07% (control) versus 33.12% (alcohol). However, adding CR, exercise, or both did not significantly lower caspase-3 levels compared to the alcohol group alone (p > 0.05), with averages of 33.66%, 36.41%, and 42.12%, respectively. These results suggest that calorie restriction and exercise or combination of both do not effectively mitigate apoptosis induced by alcohol exposure. Alcohol appears to override any metabolic interventions, perpetuating apoptosis through oxidative stress and mitochondrial dysfunction.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507003331	T171734	T1717342025	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1717342025

Publisher

: Prodi Magister Ilmu Biomedik, Fakultas Kedokteran Universitas Sriwijaya., 2025

Collation

xvi, 106 hlm., ilus., tab.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

610.280 7

Content Type

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Media Type

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Carrier Type

-

Edition

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Subject(s)

Biomedik

Specific Detail Info

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Statement of Responsibility

EM

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