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ISOLASI DAN KARAKTERISASI KOLAGEN SEBAGAI POTENSI BAHAN PRODUK ALAMI LAUT DARI FILUM ECHINODERMATA DI PERAIRAN PAHAWANG, LAMPUNG

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Penilaian

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Holothuria atra, Acanthaster planci, and Culcita novaeguineae as part of the Echinodermata phylum have the potential as a source of natural collagen that can be utilized in pharmacology. Collagen has potential bioactivities that act as antiaging, antioxidant, anti-inflammatory, and antimicrobial. This study aims to isolate and characterize collagen from the Echinodermata phylum in revealing the potential of marine natural products. Samples were taken from the neritic zone of Pahawang Island, Lampung. Antioxidant activity assessed through DPPH assay showed very strong activity (IC50 < 50 µg/ml) for all samples, with IC50 values of 10.84 µg/ml (H. atra), 21.78 µg/ml (A. planci), and 4.43 µg/ml (C. novaeguineae). LC-MS analysis identified major amino acids, including Glutamic acid, Aspartic acid, Valine, Leucine, Lysine, Isoieucine, Alanine, Glycine, Proline, Threonine, Serine, Methionine, Phenylalanine, Histidine, and Arginine, which were confirmed the existence of their compound structures by ¹³C carbon NMR signal analysis. FTIR spectroscopy detected typical collagen functional groups, including Amides I, II, III, and A. The results of molecular docking of Glutamic acid ligand (B:GLU921) to NMDA receptor (PDB:4PE5) yielded an affinity energy (ΔG) of - 5.25 kcal/mol and a Ki value of 142.02 μm, indicating its role as an endogenous agonist and potential NMDA receptor modulator. These findings provide important information in the disclosure and development of marine-derived natural products for therapeutic applications. Keywords: Antioxidant, Characterization, Collagen, Echinodermata, Molecular Docking

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507002975	T173803	T1738032025	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1738032025

Publisher

: Prodi Ilmu Kelautan, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2025

Collation

xv, 48 hlm.: Ilus., tab.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

551.407

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Kelautan

Specific Detail Info

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Statement of Responsibility

EM

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