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Image of DNA BARCODE IKAN TILAN (Mastacembelus sp.) ASAL SUNGAI MUSI DAN SUNGAI ENIM BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI)

Skripsi

DNA BARCODE IKAN TILAN (Mastacembelus sp.) ASAL SUNGAI MUSI DAN SUNGAI ENIM BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI)

Ningsih, Rezky Amanda - Personal Name;

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Spiny eel (Mastacembelus sp.) is a freshwater fish that is distributed on the islands of Java, Sumatera and Kalimantan. Mastacembelus erythrotaenia has been found in the Musi River and Mastacembelus unicolor has been found in the Enim River, the two species have different colors and patterns on their bodies. Therefore, it is necessary to study the genetic characteristics of these two species. This research aims to obtain the quantity and quality of spiny eel DNA, determine the COI gene sequence of spiny eel mitochondrial DNA and the percentage of similarities between spiny eel and other fish in GenBank data, as well as determine the water quality in the spiny eel habitat. This research was carried out in February to August 2023. The methods used in barcode were DNA extraction, DNA amplification, electrophoresis and sequencing of the COI gene region. The PCR product was obtained from the amplification with an annealing temperature of 49.2°C for 45 seconds in 31 cycles. The results showed that the DNA quantity value of spiny eel from the Musi and Enim Rivers ranged from 55.1-131.9 ng μL-1, the absorption ratio of A260/A280 from 1.64-1.98 and A260/230 from 1.60-2.73. The sequence of PCR products produced nucleotide lengths from 207-388 bp. The percentage of similarities between spiny eel and other fish in GenBank data cannot be determined. Transparency values ranged from 21.25-42 cm, dissolved oxygen (DO) from 3.52-7.66 mg L-1, current velocity from 0.14-0.47 m s-1, alkalinity from 20-40 mg L-1, pH from 6.57-7.6, temperature from 25.2-28.9°C, total dissolved solid (TDS) from 11-15 mg L-1, ammonia from 0.20-0.59 mg L-1.


Availability
Inventory Code Barcode Call Number Location Status
2407001513T137752T1377522023Central Library (References)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T1377522023
Publisher
Indralaya : Prodi Teknologi Hasil Perikanan, Jurusan Perikanan, Fakultas Pertanian., 2023
Collation
xiii, 36 hlm.; ilus.; 29 cm
Language
Indonesia
ISBN/ISSN
-
Classification
639.310 7
Content Type
Text
Media Type
unmediated
Carrier Type
-
Edition
-
Subject(s)
Sungai Musi
Prodi Teknologi Hasil Perikanan
Specific Detail Info
-
Statement of Responsibility
KA
Other version/related

No other version available

File Attachment
  • DNA BARCODE IKAN TILAN (Mastacembelus sp.) ASAL SUNGAI MUSI DAN SUNGAI ENIM BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI)
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