Skripsi

PREPARASI DAN UJI AKTIVITAS ANTI NEFROLITHIASIS NANOSUSPENSI FRAKSI DAUN MELINJO (Gnetum gnemon L.) TERHADAP TIKUS PUTIH JANTAN TERINDUKSI ETILEN GLIKOL

---

Penilaian

---

Gnetum gnemon leaves contained flavonoids that had the potential to dissolve kidney stones by increasing urinary citrate excretion and diuresis. The low bioavailability of fractions required nanoparticle dosage forms as an option to increase the effectiveness of the delivery system. This study aimed to determine the percentage of nanosuspension entrapment efficiency used for in vivo tests and the effect of melinjo leaf fraction nanosuspension on calcium, creatinine, serum urea, and kidney histopathology levels in kidney stone rats. Nanosuspensions with three variations of chitosan 0.1%, 0.3%, and 0.75% were made using the ionic gelation method. The antrinefrolithiasis activity test was carried out in vivo on male rats induced by ethylene glycol and ammonium chloride. The positive control was given Batugin elixir, the negative control was given a placebo nanosuspension, and the test group was given a nanosuspension of ethanol and ethyl acetate fractions of melinjo leaves. The best formula was obtained from formula I with an average particle size of 191.77 nm, zeta potential -35.05 mV, PDI of 0.4018, and %EE of 96.7488% for ethanol fraction nanosuspension, and a particle size of 198.87 nm, zeta potential -35.15 mV, PDI of 0.3875, and %EE of 97.9526% for ethyl acetate fraction nanosuspension. The results showed a decrease in the average levels of creatinine, serum urea, and kidney calcium and an increase in urine pH in the melinjo leaf fraction nanosuspension test group. Duncan's statistical analysis showed no significant difference between the melinjo leaf fraction nanosuspension and the positive and normal groups (p>0.05). Ethyl acetate fraction nanosuspension was more effective in dissolving kidney stones than ethanol fraction nanosuspension based on histopathology results. Keywords : antinephrolithiasis, ethylene glycol, Gnetum gnemon L, kidney stones, nanosuspension.

---

Availability

Inventory Code	Barcode	Call Number	Location	Status
2507001249	T167801	T1678012025	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

-

Call Number

T1678012025

Publisher

Indralaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam., 2025

Collation

xvii, 170 hlm.; ilus.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

-

Classification

582.120 7

Content Type

Text

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Tanaman Obat--Ekstrak Daun Melinjo

Specific Detail Info

-

Statement of Responsibility

MI

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment