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PENGARUH NATRIUM HIPOKLORIT (NaOCl) TERHADAP TINGKAT KONTAMINASI EKSPLAN HELAIAN DAUN BUAH DURIAN (Durio zibethinus) SECARA KULTUR IN-VITRO

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Penilaian

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This study was aimed to evaluate the effect of sodium hypochlorite for sterilizing young leaf explants of durian plants (Durio zibethinus) in in-vitro culture. This research was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Sriwijaya University, Bukit Lama, Kec. Ilir Barat I, Palembang City, South Sumatra with location coordinates 2 59'23.4"S; 104 43'53.4"E from June to September 2024. This sterilization treatment procedure used 5 combinations of sterilant materials, each consisting of 10 culture bottles with 3 replicates resulted in total of 150 experimental bottles. The treatments were the combinations of liquid detergent, bactericide, fungicide, NaOCl, and 70% alcohol with different soaking durations. The design used was parametric descriptive analysis by calculating the percentage of explants contaminated with bacteria and fungi, the percentage of media contaminated with bacteria and fungi, and the percentage of live explants. It was resulted that sterilization of leaf explants (lamina) of durian plants using the P5 treatment procedure (Liquid Detergent for 3' + Streptomycin Sulfate 0.2% for 10' + Benomyl 0.2% for 10' + NaOCl 1.5% for 5 ' + Alcohol 70% for 5') showed the most significant results with 53% of live explants percentase and only 23% of contamination in both explants and media.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2507000105	T163945	T1639452024	Central Library (REFERENCE)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1639452024

Publisher

Indralaya : Prodi Agronomi, Jurusan Budidaya Pertanian, Fakultas Pertanian Uniersitas Sriwijaya., 2024

Collation

xiv, 46 hlm., ilus., tab.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

630.07

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Agronomi

Specific Detail Info

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Statement of Responsibility

EM

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