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PEMISAHAN FRAKSI KOLOM DARI FRAKSI ETIL ASETAT DAUN KERSEN (MUNTINGIA CALABURA L.) DAN UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH

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Penilaian

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Kersen leaves (Muntingia calabura L.) have been proven to have antioxidant properties, both in the form of crude extracts and fractions. The ethyl acetate fraction of kersen leaves separated by vacuum liquid column chromatography has strong antioxidant activity (IC50 43,40 µg/mL). This research is a continuation of the previous research by further separating the fraction of vacuum liquid column results from the ethyl acetate fraction of kersen leaves using gravity column chromatography. This study is useful to determine the antioxidant activity of the results of column fraction separation of kersen leaves and identify specific compounds to further determine the ability of antioxidants in inhibiting free radicals. Fraction separation was carried out by column chromatography on silica gel with eluent of increasing polarity for two separations to obtain more specific compounds. Compound monitoring used thin layer chromatography technique to determine the chromatogram pattern. Each column fraction was tested for antioxidant activity using the 1,1 diphenyl-2-picryl hydrazyl (DPPH) method and the active fraction of antioxidant compounds was identified using IR spectroscopy techniques. The results of the separation of the column fractions showed that the antioxidant activity of column fraction D increased, while column fraction E decreased in activity. The results of this test showed that the final column fraction in the advanced separation FD4 (2) had the highest level of antioxidant activity with an IC50 value of 17,363 µg/mL. This indicates the specific compound concentrated in fraction form has high antioxidant potential. IR spectroscopy identification results concluded that the secondary metabolite compounds that were successfully separated were flavonoids as much as 77,97 mg.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2407000966	T138972	T1389722024	Central Library (References)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1389722024

Publisher

Inderalaya : Prodi Ilmu Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam., 2024

Collation

xviii, 104 hlm.; tab.; 29 cm.

Language

Indonesia

ISBN/ISSN

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Classification

582.120 7

Content Type

Text

Media Type

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Carrier Type

-

Edition

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Subject(s)

Prodi Ilmu Farmasi
Tanaman Obat--Daun Kersen

Specific Detail Info

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Statement of Responsibility

MI

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