Skripsi

EKSPLORASI FUNGI PENDEGRADASI SELULOSA LIMBAH TANDAN KOSONG KELAPA SAWIT (TKKS)

---

Penilaian

---

Oil palm empty fruit bunches (EFBF) waste is a waste that takes a long time to degrade, this is due to the high cellulose content in EFB waste. Cellulose is a hydrolyzed polysaccharide to produce cellobiose and glucose monomers. Cellulase can be produced by plants, yeast, bacteria and cellulolytic fungi. Fungi have the ability to decompose cellulose found in dead plant tissues into simpler compounds due to their ability to produce lignocellulolytic enzymes. Cellulolytic fungi are microorganisms capable of degrading cellulose by producing cellulase enzymes. Cellulase is an enzyme complex that plays a role in the hydrolysis of cellulose consisting of endoglucanase, ecoglucanase, and cellobiase. This study aims to determine the genus of fungi that have high cellulase activity values isolated from OPEFB waste. The research was conducted from January to May 2023, taking place at the Microbiology Laboratory, as well as the Genetics and Biotechnology Laboratory, Department of Biology, FMIPA, Sriwijaya University, Indralaya. The stages of the research were isolation and purification of fungi, qualitative selection of cellulolytic function, preparation of crude extracts of cellulase enzymes, preparation of glucose standard curves, quantitative testing of cellulase enzyme activity using the DNS method, characterization and identification of high cellulase activity values. The results showed that 8 out of 11 fungi isolates had the ability to produce cellulase enzymes. Isolates TS2B1, TS1L1, TS3L2 and TS3L3 had high cellulase activity values respectively 5.7 (U/ml), 6.5 (U/ml), 6.1 (U/ml), 5.8 (U/ ml) based on cellulase enzyme activity test with DNS method. Isolates TS2B1, TS1L1, TS3L2 and TS3L3 had high cellulase activity values respectively 5.7 (U/ml), 6.5 (U/ml), 6.1 (U/ml), 5.8 (U/ ml) based on cellulase enzyme activity test with DNS method. Isolate TS2B1 was identified as the genus Penicillium, isolates TS1L1 and TS3L3 were identified as the genus Fusarium and isolate TS3L2 was identified as the genus Trichoderma

---

Availability

Inventory Code	Barcode	Call Number	Location	Status
2307002890	T123482	T1234822023	Central Library (Referens)	Available but not for loan - Not for Loan

Detail Information

Series Title

-

Call Number

T1234822023

Publisher

Inderalaya : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2023

Collation

xv, 55 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

-

Classification

570.707

Content Type

Text

Media Type

unmediated

Carrier Type

-

Edition

-

Subject(s)

Jurusan Biologi
Riset dan Topik Terkait di Bidang Biologi

Specific Detail Info

-

Statement of Responsibility

SEPTA

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment