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OPTIMASI AKTIVITAS ENZIM SELULASE YANG DIHASILKAN OLEH BAKTERI SELULOLITIK Y

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Penilaian

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Cellulase enzymes break β-1,4 glycosidic bonds in cellulose, cellodextrin, cellobiose and other cellulose derivatives. This research aimed to determine the highest cellulase enzyme activity of several bacterial isolates using the DNS method and to study the optimum conditions of pH, temperature, substrate and metal ion concentrations of the cellulase enzyme activity. This research used a non-factorial Completely Randomized Design (CRD) with four treatment factors repeated three times. The parameter observed was the amount of reducing sugar released in cellulase enzyme activity. The results showed cellulase enzyme activity was measured using the DNS method for 20 isolates of cellulolytic bacteria. Two bacterial isolates had the highest enzyme activity, sample code D 10-6 (39) of 10.20 U/mL and D 10-6 (44) of 8.86 U/mL. This study used the second-highest bacterial isolate, sample D(10-6) 44. The optimum conditions for cellulase activity from bacterial isolate D (10-6) 44 were at pH 7 with an activity value of 7.03 U/mL, temperature 30oC with Enzyme activity was 6.05 U/mL, substrate concentration was 0.5% with an enzyme activity value of 85.03 U/mL and the addition of FeCl2 metal ion with an enzyme activity value of 100.45 U/mL, FeCl2 metal ion acts as an activator in cellulase activity, while the addition of metal ions MgCl2, CaCl2, ZnCl2, and CuCl2 acts as an inhibitor in cellulase activity.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2307004186	T104785	T1047852023	Central Library (Referens)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T1047852023

Publisher

Inderalaya : Prodi Teknologi Hasil Pertanian, Fakultas Pertanian Universitas Sriwijaya., 2023

Collation

xvi, 38 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

632.07

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Prodi Teknologi Hasil Pertanian
Kerusakan pada Tanaman Pertanian

Specific Detail Info

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Statement of Responsibility

SEPTA

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