Skripsi

POTENSI BAKTERI SELULOLITIK DARI TANDAN KOSONG KELAPA SAWIT (Elaeis guineensis Jacq.)

---

Penilaian

---

Cellulose is a very long chain polymer of glucose that supports plant structure. Cellulase enzyme is an enzyme that has the ability to degrade cellulose with its main product, glucose. Cellulase enzymes can be produced by cellulolytic bacteria isolated from various types of substrates, one of which is empty oil palm bunches (Elaeis guineensis Jacq.). Oil palm empty bunches have a cellulose content of 45.95%, hemicellulose 16.49%, and lignin 22.84%. Cellulose as the largest component in empty oil palm bunch waste that can be used as a substrate for the growth of cellulolytic bacteria to produce cellulase enzymes. The process of cellulose degradation can be carried out by enzymatic means with the help of microorganisms. Cellulolytic bacteria are microorganisms that are able to produce cellulase enzymes. Cellulolytic bacteria function to hydrolyze cellulose into a simpler product, glucose. This study aims to determine the genus of bacteria that have high cellulase activity isolated from empty oil palm bunches. This research will be carried out from January to March 2023, located at the Microbiology Laboratory, as well as the Genetics and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya. The stages of research carried out are isolation and purification of cellulolytic bacteria, qualitative cellulase enzyme activity test, inoculum making, crude extract cellulase enzyme production, glucose standard curve making, cellulase activity test with DNS method, characterization and identification of cellulolytic bacteria that have high cellulase activity value. The conclusion of this study is that cellulolytic bacterial isolates from empty oil palm bunches have the ability to produce cellulase enzymes, which is as many as 11 isolates from 28 bacterial isolates. TLA16, TLB5, and TLA14 isolates have high cellulase activity values of 3.2 U/ml, 1.9 U/ml, and 1.8 U/ml respectively based on quantitative tests using DNS methods. TLA16 and TLB5 isolates were identified as the genus Lactobacillus and TLA14 isolates were identified as the genus Carnobacterium.

---

Availability

Inventory Code	Barcode	Call Number	Location	Status
2307003330	T123400	T1234002023	Central Library (Referens)	Available

Detail Information

Series Title

-

Call Number

T1234002023

Publisher

Indralaya : Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2023

Collation

xv, 67 hlm.; Ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

-

Classification

579.307

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Bakteri
Jurusan Biologi

Specific Detail Info

-

Statement of Responsibility

PITRIA

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment