Skripsi

UJI AKTIVITAS ENZIM AZOREDUKTASE EKSTRASELULER DARI BAKTERI AEROMONAS JANDAEI

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Penilaian

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Extracellular azoreductase enzyme activity has been tested from Aeromonas jandaei bacteria. Extract of azoreductase enzyme from A. jandaei bacteria was obtained by centrifugation of liquid culture on NB media which was incubated for 2x24 hours at 37C in an incubator shaker. The results of enzyme fractionation with the highest specific activity were used to determine the optimum conditions for the extracellular azoreductase enzyme from A. jandaei bacteria with variations in incubation time, incubation temperature, pH and substrate concentration. From the activity substrate concentration, enzyme kinetics were calculated, namely Vmax and Km. The crude extract of the azoreductase enzyme had an activity of 0.025 U/mL and a specific enzyme activity of 0.00139 U/mg. The results of the fractionation obtained the highest specific enzyme activity in fraction 3 with a saturation level of 40-60% ammonium sulfate with a specific enzyme activity value of 0.0844 U/mg. Extracellular azoreductase enzyme from A. jandaei bacteria has optimum conditions at incubation time of 5 minutes, pH 7 temperature 37ºC and substrate concentration of 70µM with enzyme activity of 0.1017 U/mL. Calculation of the enzyme kinetic data obtained a Vmax value of 0.1025 U/mg and a Km value of 0.6886 M.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2307005830	T67558	T675582022	Central Library (Referens)	Available

Detail Information

Series Title

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Call Number

T675582022

Publisher

Indralaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2023

Collation

xvii, 58 hlm.; Ilus.; 21 cm

Language

Indonesia

ISBN/ISSN

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Classification

660.634 07

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Jurusan Kimia
Enzim Azoreduktase

Specific Detail Info

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Statement of Responsibility

PITRIA

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