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Image of ISOLASI SENYAWA BIOAKTIF DARI JAMUR ENDOFITIK DAUN SUNGKAI (Peronema canescens Jack.)

Skripsi

ISOLASI SENYAWA BIOAKTIF DARI JAMUR ENDOFITIK DAUN SUNGKAI (Peronema canescens Jack.)

Nasution, Sakinah Salman Ahmad - Personal Name;

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Penilaian anda saat ini :  

Sungkai leaves (Peronema canescens Jack.) has been used by the community for a long time as a traditional medicine for fever, stomachache, mouth antiseptic, and toothache medicine. This research was conducted to isolate and identify bioactive compounds from endophytic fungi of sungkai leaves. The research was started by isolating and purifying endophytic fungi from fresh sungkai leaves, then identified morphologically. Then the endophytic fungi were cultivated for 4 weeks on PDB media and extracted to obtain a concentrated extract of ethyl acetate. The extract obtained will be tested for antioxidants using the DPPH method. Extracts with the highest antioxidant potential will be isolated by chromatographic technique and the endophytic fungi will be identified molecularly. The pure compound obtained will be determined spectroscopically for its structure.The results of the isolation and purification of endophytic fungi obtained five isolates of endophytic fungi with different species. The five fungal isolates were cultivated and extracted to obtain a concentrated extract of ethyl acetate. The ethyl acetate extract was tested for antioxidant activity and it was found that the SD3 isolate had the highest activity (IC50 = 22.32 μg/mL). Based on the phylogenetic tree construction, the SD3 endophytic fungus is Colletotrichum gloeosporioides. The results of isolation of the SD3 extract using column chromatography and analysis using thin layer chromatography obtained pure compounds in the form of compounds with an aromatic group that has a para-substituted benzene ring, ester and hydroxyl groups in the form of a white solid (74 mg) which has strong antioxidant activity with IC50 = 20. 87 μg/mL.


Availability
Inventory Code Barcode Call Number Location Status
2307000966T85576T855762023Central Library (Referens)Available
Detail Information
Series Title
-
Call Number
T855762023
Publisher
Indralaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2023
Collation
xv, 46 hlm.; ilus 21 cm
Language
Indonesia
ISBN/ISSN
-
Classification
669.607
Content Type
-
Media Type
-
Carrier Type
-
Edition
-
Subject(s)
Jurusan Kimia
Antibakteri Senyawa
Specific Detail Info
-
Statement of Responsibility
PITRIA
Other version/related

No other version available

File Attachment
  • ISOLASI SENYAWA BIOAKTIF DARI JAMUR ENDOFITIK DAUN SUNGKAI (Peronema canescens Jack.)
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