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DNA BARCODING HORSESHOE CRAB ASAL PERAIRAN KUALA TUNGKAL, JAMBI DAN PESISIR BANYUASIN, SUMATERA SELATAN

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Penilaian

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Conservation is one solution to preserve the horseshoe crab, which has been categorized as an endangered biota. With DNA barcode, horseshoe crab can be conserved based on its species. This study aims to identify and determine the relationship between horseshoe crab by using DNA barcode. This research carried out from July to November 2021 in the Kuala Tungkal Waters, Jambi and Banyuasin Coastal, South Sumatera with 13 station points by taking 23 horseshoe crabs. In situ measurements consist of temperature, salinity, pH, DO and depth. Sample preparation was carried out by extracting genomic DNA using the GeneAid Kit, DNA amplification using the PCR Thermal Cycler and DNA visualization using Electrophoresis. The data were analyzed with BioEdit and MEGA 11, then identified with the NCBI website. The identification result using DNA barcode in Banyuasin Coastal, South Sumatera consisted of C. rotundicauda and T. gigas species, while in Kuala Tungkal Waters, Jambi consisted of C. rotundicauda species. Based on the phylogenetic tree, it can be seen that there is a relationship between C. rotundicauda at the two research sites, which indicates the occurrence of gene from one place to another. The origin of genetic diversity of the T. gigas horseshoe crab in Banyuasin comes from Singapore, C. rotundicauda in Banyuasin comes from Johor, Korea and China. Meanwhile, C. rotundicauda in Kuala Tungkal came from Johor and Korea.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2207003004	T75266	T752662022	Central Library (Referens)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T752662022

Publisher

Inderalaya : Jurusan Ilmu Kelautan, Fakultas Matematika Dan Ilmu Pengetahuan Alam., 2022

Collation

xviii, 45 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

577.607

Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

Jurusan Ilmu Kelautan
Ekologi Perairan dan Ekologi Air Tawar

Specific Detail Info

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Statement of Responsibility

SEPTA

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