Skripsi

KINETIKA HIDROLISIS ENZIMATIK DARI SELULOSA SEKAM PADI YANG DIBERI PRAPERLAKUAN HIDROGEN PEROKSIDA-AQUEOUS AMMONIA

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Penilaian

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Rice husks contain cellulose as a raw material for manufacturing second-generation bioethanol. Cellulose from pre-treated rice husks was converted into reducing sugars through enzymatic hydrolysis using enzymes derived from Aspergillus niger. This study aims to determine the effect of variations in concentration, hydrolysis time and kinetics of enzymatic hydrolysis at enzyme concentrations of 10, 15, and 20% (v/w) and hydrolysis times of 5, 10, 15, 20, and 25 h. The results showed that cellulose was hydrolyzed to form reducing sugars. The CMCase activity and FPase activity reached 548.940 and 314.892 U mL-1, respectively, much higher than most previous reports on this genus. From the calculation of the reaction rate using the Michaelis–Menten kinetic model, the value of the Michaelis constant ranges from 0.001 to 0.0007, and the maximum rate is 1.3 × 10-7 to 2.7 × 10-7 Mol L-1 s-1. The highest reducing sugar concentration was obtained (1.80 g L-1) at an enzyme concentration of 20% (v/w) and a hydrolysis time of 25 h with an optimal condition pH of 5.

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2207004857	T83197	T831972022	Central Library (Referens)	Available

Detail Information

Series Title

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Call Number

T831972022

Publisher

Indralaya : Prodi Magister Teknik Kimia, Fakultas Teknik Universitas Sriwijaya., 2022

Collation

xvi, 63 hlm.; ilus.; 29 cm

Language

Indonesia

ISBN/ISSN

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Classification

541.370 7

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

teknik kimia
Elektrokimia dan Magnetokimia

Specific Detail Info

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Statement of Responsibility

MURZ

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