The Sriwijaya University Library

  • Home
  • Information
  • News
  • Help
  • Librarian
  • Login
  • Member Area
  • Select Language :
    Arabic Bengali Brazilian Portuguese English Espanol German Indonesian Japanese Malay Persian Russian Thai Turkish Urdu

Search by :

ALL Author Subject ISBN/ISSN Advanced Search

Last search:

{{tmpObj[k].text}}
Image of PENGARUH pH DAN TEMPERATUR TERHADAP AKTIVITAS ENZIM SELULASE YANG DIISOLASI DARI Bakteri Enterobacter cloacae DAN Bakteri Klebsiella pneumonia YANG DIDAPAT DARI SALURAN PENCERNAAN RAYAP

Text

PENGARUH pH DAN TEMPERATUR TERHADAP AKTIVITAS ENZIM SELULASE YANG DIISOLASI DARI Bakteri Enterobacter cloacae DAN Bakteri Klebsiella pneumonia YANG DIDAPAT DARI SALURAN PENCERNAAN RAYAP

Sulistiawati, Rolis - Personal Name;

Penilaian

0,0

dari 5
Penilaian anda saat ini :  

Termites are pests that can cause damage to various types of wood, buildings and plants. Termites can digest cellulose because their intestines contain cellulolytic bacteria. Cellulolytic bacteria from termite digestion have been isolated, two of which are Enterobacter Clocoae and Klebsiella Pneumoniae. This study aimed to study the effect of pH and temperature on cellulase enzyme activity isolated from Enterobacter Clocoae bacteria and Klebsiella Pneumoniae bacteria obtained from the digestive tract of termites. Crude extract of cellulase enzyme was obtained extracellularly from Enterobacter Clocoae and Klebsiella Pneumoniae bacterial cultures. Extracellular enzymes were obtained from the process of centrifugation of bacterial liquid cultures at a speed of 15,000 rpm at 4 oC for 5 minutes. The crude extract enzyme was then fractionated with ammonium sulfate. The fraction with the highest enzyme activity was used to determine the effect of pH and temperature on cellulase enzyme activity. Enzyme activity was determined using CMC substrate and the result of hydrolysis in the form of sugar was determined by the 3,5- dinitrosalicylic acid (DNS) method and measured by UV-Vis spectrophotometer. The results showed that the extracellular enzyme activity of the crude extract obtained from Enterobacter cloacae was 0.001 U/mL, while the extracellular enzyme activity obtained from Klebsiella pneumoniae was 0.0007 U/mL. The highest enzyme activity of Enterobacter cloacae was obtained in fraction 2 with cellulase enzyme activity of 0.017 U/mL, while the activity of cellulase enzymes produced from Klebsiella pneumoniae which had the highest activity was found in fraction 3 with enzyme activity of 0.012 U/mL. Cellulase enzyme activity of Enterobacter cloacae optimum at pH 5 resulted in enzyme activity of 0.020 U/mL and optimum at 35oC with cellulase enzyme activity of 0.018 U/mL. Cellulase enzyme activity of Klebsiella pneumoniae optimum at pH 8 resulted in enzyme activity of 0.016 U/mL and optimum at 30oC with cellulase enzyme activity of 0.018 U/mL. Based on the data obtained, it can be concluded that Enterobacter cloacae has the best conditions at pH 5 and a temperature of35oC, while Klebsiella pneumoniae has the best conditions at pH 8 and a temperature of 30oC.


Availability
Inventory Code Barcode Call Number Location Status
2207003414T75113T751132022Central Library (Referens)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T751132022
Publisher
Inderalaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2022
Collation
ix 37 hlm.; ilus.; 29 cm
Language
Indonesia
ISBN/ISSN
-
Classification
547.707
Content Type
-
Media Type
-
Carrier Type
-
Edition
-
Subject(s)
Jurusan Kimia
Senyawa Makromolekul dan yang Berkaitan
Specific Detail Info
-
Statement of Responsibility
SEPTA
Other version/related

No other version available

File Attachment
  • PENGARUH pH DAN TEMPERATUR TERHADAP AKTIVITAS ENZIM SELULASE YANG DIISOLASI DARI Bakteri Enterobacter cloacae DAN Bakteri Klebsiella pneumonia YANG DIDAPAT DARI SALURAN PENCERNAAN RAYAP
Comments

You must be logged in to post a comment

The Sriwijaya University Library
  • Information
  • Services
  • Librarian
  • Member Area

About Us

As a complete Library Management System, SLiMS (Senayan Library Management System) has many features that will help libraries and librarians to do their job easily and quickly. Follow this link to show some features provided by SLiMS.

Search

start it by typing one or more keywords for title, author or subject

Keep SLiMS Alive Want to Contribute?

© 2025 — Senayan Developer Community

Powered by SLiMS
Select the topic you are interested in
  • Computer Science, Information & General Works
  • Philosophy & Psychology
  • Religion
  • Social Sciences
  • Language
  • Pure Science
  • Applied Sciences
  • Art & Recreation
  • Literature
  • History & Geography
Icons made by Freepik from www.flaticon.com
Advanced Search