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ANALISIS KADAR NIKOTIN PADA TEMBAKAU (NICOTIANA TABACUM L) MENGGUNAKAN METODE GC-MS

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Penilaian

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Nicotine is an alkaloid compound found in tobacco. Nicotine content is included in the tobacco quality parameters. The nicotine content is 1-3% based on the quality requirements according to SNI 0612-1989-A. This study aims to determine nicotine levels in mol and Ranau tabacum varieties using gas chromatography-mass spectrometry (GCMS). The study begins with a phytochemical study of alkaloids using the Dragendorff's reagent. In addition, the alkaloid test was performed by thin layer chromatography (TLC) using ethyl acetate: methanol (6: 4; 7: 3; 8: 2) in the stationary and mobile phases (eluents) of silica gel. bottom. Dragendorff's. The spot is displayed. Next, the nicotine level is measured by the GCMS method by extracting the sample with methanol. Under GCMS operating conditions, a TG5MS column (30m x 0.25mm) 0.25µm thick was used and helium was used as the mobile phase at a flow rate of 1mL / min. The results of the phytochemical screening showed a positive test for alkaloids in the presence of a red brick-colored precipitate. TLC tests also showed the presence of alkaloid compounds with red brick stains. GCMS results showed the presence of several alkaloid compounds such as nicotine, nornicotine and anatabine. The results of the tobacco extract test showed that the molar and Ranau varieties had nicotine levels of 0.02 g and 0.06 g respectively. The nicotine content produced in tobacco samples is very low and is not included in the quality requirements of SNI 0612-1989-A

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2107004696	T58499	T584992021	Central Library (REFERENCES)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T584992021

Publisher

Inderalaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2021

Collation

xvii, 50 hm. :ilus. ; 21 cm

Language

Indonesia

ISBN/ISSN

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Classification

362.296 07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Nikotin - Metode GC-MS

Specific Detail Info

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Statement of Responsibility

AA

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