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Image of ANALISIS KADAR NIKOTIN PADA TEMBAKAU (NICOTIANA TABACUM L) MENGGUNAKAN METODE GC-MS

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ANALISIS KADAR NIKOTIN PADA TEMBAKAU (NICOTIANA TABACUM L) MENGGUNAKAN METODE GC-MS

Ayu, Demi - Personal Name;

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Nicotine is an alkaloid compound found in tobacco. Nicotine content is included in the tobacco quality parameters. The nicotine content is 1-3% based on the quality requirements according to SNI 0612-1989-A. This study aims to determine nicotine levels in mol and Ranau tabacum varieties using gas chromatography-mass spectrometry (GCMS). The study begins with a phytochemical study of alkaloids using the Dragendorff's reagent. In addition, the alkaloid test was performed by thin layer chromatography (TLC) using ethyl acetate: methanol (6: 4; 7: 3; 8: 2) in the stationary and mobile phases (eluents) of silica gel. bottom. Dragendorff's. The spot is displayed. Next, the nicotine level is measured by the GCMS method by extracting the sample with methanol. Under GCMS operating conditions, a TG5MS column (30m x 0.25mm) 0.25µm thick was used and helium was used as the mobile phase at a flow rate of 1mL / min. The results of the phytochemical screening showed a positive test for alkaloids in the presence of a red brick-colored precipitate. TLC tests also showed the presence of alkaloid compounds with red brick stains. GCMS results showed the presence of several alkaloid compounds such as nicotine, nornicotine and anatabine. The results of the tobacco extract test showed that the molar and Ranau varieties had nicotine levels of 0.02 g and 0.06 g respectively. The nicotine content produced in tobacco samples is very low and is not included in the quality requirements of SNI 0612-1989-A


Availability
Inventory Code Barcode Call Number Location Status
2107004696T58499T584992021Central Library (REFERENCES)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T584992021
Publisher
Inderalaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya., 2021
Collation
xvii, 50 hm. :ilus. ; 21 cm
Language
Indonesia
ISBN/ISSN
-
Classification
362.296 07
Content Type
Text
Media Type
-
Carrier Type
-
Edition
-
Subject(s)
Nikotin - Metode GC-MS
Specific Detail Info
-
Statement of Responsibility
AA
Other version/related

No other version available

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  • ANALISIS KADAR NIKOTIN PADA TEMBAKAU (NICOTIANA TABACUM L) MENGGUNAKAN METODE GC-MS
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