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PATOGENESITAS JAMUR ENTOMOPATOGEN YANG DIPERKAYA DENGAN EKSTRAK KOMPOS TERHADAP LARVA Spodoptera frugiperda DAN POTENSINYA SEBAGAI PEMACU PERTUMBUHAN BIBIT JAGUNG
Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is an important pest on maize that causes damage to maize crops in Indonesia. The loss caused by this pest is about a third of the corn harvest. This pest spreads rapidly in several locations in Indonesia. Control using endophytic fungi is more effective because the fungi are associated with plant tissues and can stimulate plant growth. This study aims to determine the level of pathogenicity of entomopathogenic fungi with compost extract inoculated through seeds and leaves. This study was designed according to a factorial randomized block design with two factors, namely the isolate factor and the method of inoculation with isolate codes A, B, C, and D (control with EKKU) and replicated 3 times. The method used in this study, namely the test of fungal colonization on corn leaves and the potential test of entomopathogenic fungi with compost extract which was proven to be endophytic against S. frugiperda larvae. The variables observed in this study were conidia density, conidia viability, leaf area eaten, larval body weight, larval droppings weight, larval mortality, LT50, LT95, percentage of pupae appearing, normal and abnormal pupae, body length and wing span of imago, lifespan of imago, the number of eggs laid, the number of eggs hatched, the number of larvae that did not hatch, the effect of entomopathogenic fungi with compost extract on the growth of maize seedlings, and the effect of entomopathogenic fungi with compost extracts on the environment in hydroponic media. The results showed that 3 isolates were proven to be endophytic, namely A, B, and C through seed inoculation and leaf inoculation. The highest percentage of endophytic fungal colonization was in isolates A and C, which was 53.33%. The results showed the highest spore density, the highest percentage of viability and the highest mortality rate were obtained from larvae treated with isolate C with a mortality percentage of 20.67% and the shortest LT50 and LT95 were 17.51 and 28.45 days and the lowest mortality was in the larvae inoculated with the fungal isolate code B, which was 14.00 % with LT50 and LT95 18.21 days and 29.16 days. The method of seed inoculation had a higher mortality of 17.00%, with LT50 and LT95 19.31 days and 27.51 days. Meanwhile, leaf inoculation had a mortality of 13.33% with LT50 and LT95 21.29 days and 29.98 days. The lowest produced eggs and hatched eggs were obtained from the treatment of isolate D with an average number of eggs 93.50 eggs and larvae that appeared 79.83 eggs and the highest was in the control. The effect of entomopathogenic fungi with compost extract on the growth of maize seedlings after 7 days after planting and 14 days after planting had better growth and was significantly different from the control. The highest corn seedling growth was found in isolate C and the lowest was in control, while the method of inoculation that had higher growth of corn seedlings was by inoculation through seeds.
Inventory Code | Barcode | Call Number | Location | Status |
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2107004809 | T62989 | T629892021 | Central Library (Referens) | Available but not for loan - Not for Loan |
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