Skripsi

ISOLASI SENYAWA METABOLIT SEKUNDER DARI DAUN TUMBUHAN LUDEIGIA PERUVIANA DAN AKTIVITAS ANTIBAKTERINYA

---

Penilaian

---

ISOLATION OF SECONDARY METABOLITES FROM Ludwigia peruviana LEAVES AND IT’S ANTIBACTERIAL ACTIVITY Patrick Ahad: Advised by Dr. Ferlinahayati, M.Si and Dr. Eliza, M.Si. Departement of Chemistry, Faculty of Mathemathics and Natural Sciences, Sriwijaya University XI + 62 pages, 22 pictures, 12 tables, 9 atachements The isolation of secondary metabolites from Ludwigia peruvina leaves along with its antibacterial activity had been done. The isolation started with maceration of dried L. peruviana leaves using methanol as the solvent, then continued with liquid-liquid partition with n-hexane and ethyl acetate. The partition yielded n-hexane fraction which then further separated using vacuum liquid chromatography and gravity column chromatography. The isolation and purification yielded 16,3 mg of white oil-like compound. The isolated compound structure then analyzed using IR, 13C-NMR and 1H-NMR spectrum and GC-MS. Based on the data and its comparison with literature data, its shown that the isolated compound was a mixture of terpenoid namely cycloartenol (a), 24-methylene-cycloartenol (b) and phytol (c). The antibacterial acitivity of methanol extract, n-hexane fraction, ethyl acetate fraction and the isolated compound were tested using agar disc diffusion method against two strain of bacteria, Eschericia coli (ATCC 25922) and Staphylococus aureus (ATCC 25923). The results showed that methanolic extract of L. peruviana leaves, n-hexane and ethyl acetate fraction managed to inhibit S. aureus growth but, unable to inhibit E. coli growth in any concentration. The isolated compound also managed to inhibit S. aureus growth but, unable to inhibit E. coli growth. From the results, its shown that methanolic extract of L. peruviana have antibacterial properties toward gram positive bacteria, S. aureus as well as the both fractions and the isolated compounds.

---

Availability

Inventory Code	Barcode	Call Number	Location	Status
2107004022	T50404	T504042021	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

-

Call Number

T504042021

Publisher

Inderalaya : Prodi Ilmu Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam., 2021

Collation

xvi, 50 hlm.; tab.; ilus.; 21 cm.

Language

Indonesia

ISBN/ISSN

-

Classification

547.707

Content Type

Text

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

Mipa Kimia
Metabolit Sekunder Daun Ludwigia Peruviana

Specific Detail Info

-

Statement of Responsibility

MI

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment