Skripsi

IDENTIFIKASI SENYAWA ORGANIK FRAKSI ENDAPAN DARI EKSTRAK METANOL BUAH TEMBESU (Fragraea fragrans Roxb) SECARA LCMS/MS DAN AKTIVITAS ANTIBAKTERI

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Penilaian

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This research was conducted to identify organic compounds in the precipitate fraction of the methanol extract tembesu fruit (Fragraea fragrans) by LCMS/MS analysis and antibacterial activity against P. acnes, S.aureus and P.aeruginosa. Tembesu fruit (Fragraea fragrans) was extracted by maceration with methanol solvent and carried out at room temperature. Antibacterial test of organic compounds of precipitate fraction of the methanol extract activity of tembesu fruit (test compound) against P. acnes, S. aureus and P. aeruginosa bacteria has also been done. Identification of those compounds was carried out by means of LCMS/MS analysis. Tembesu fruit extract was successfully precipitated as much as 4g (0,3 %) in the form of a yellow powder. Based on the LCMS/MS data analysis, there were eleven organic compounds and three of them as the major at retention time of 9,36; 9,58 and 3,91 minutes in the form of ursolic acid and it’s isomer oleanolic acid, 3-oxo-ursanaldehyde and it’s isomer 3-oxo-oleanaldehyde and erythrocentaurine. Test compound showed antibacterial activity with a Minimum Inhibitory Concentration (MIC) value of 20 mg/mL against of P. acnes bacteria, 25 mg/mL against S.aureus and P. aeruginosa bacteria. Providing a Minimum Bacteria Concentration (MBC) against P. acnes, S. aureus and P. aeruginosa of 30 mg/mL in each of tested bacteria. Keywords : Tembesu fruit, Precipitate, Antibacterial, LCMS/MS

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Availability

Inventory Code	Barcode	Call Number	Location	Status
2107003983	T50805	T508052021	Central Library (Reference)	Available but not for loan - Not for Loan

Detail Information

Series Title

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Call Number

T508052021

Publisher

Inderalaya : Prodi Ilmu Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam., 2021

Collation

xvii, 37 hlm.; tab.; 21 cm.

Language

Indonesia

ISBN/ISSN

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Classification

547.07

Content Type

Text

Media Type

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Carrier Type

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Edition

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Subject(s)

Mipa Kimia
Ektrak Metanol Buah Tembesu

Specific Detail Info

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Statement of Responsibility

MI

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