Skripsi
UJI AKTIVITAS ANTIJAMUR (Candida albicans) DAN KESTABILAN KOMBINASI EKSTRAK AIR ZAT WARNA ALAMI KULIT BUAH MANGGIS (Graclnto mangostana. L) DAN KAYU SECANG (CaeSalpinta sappan. L)
The investigation of antifungal activity and stability of combination the water extract of mangosteen fruit hull (Garcinia Mangostana. L) and secang wood (Caesalpinia Sappan. L) has been conducted. The tested demartophyta used Candida albicans. This research using difusion method to determine the antifungal activity and the stability of dye combination was tested against oxidizing agent (H202), light (UV), heating temperature and diffrent storage condition. The results of determination minimum inhibitory concentration (MIC) showed that the water extract of secang wood higher than fruit hull of mangosteen. MIC combination of extract mangosteen fruit hull with secang wood respectively, 1,95 mg/ml : 0,24 mg/ml; 0,97 mg/ml : 0,48 mg/ml; 0,48 mg/ml : 0,97 mg/ml and 0,24 mg/ml : 0,97 mg/ml. The determination for zone inhibition showed that combination of two extract is the larger than the single extract fruit hull of mangosteen and smaller than single extract secang wood. The research showed the unstability the against oxidizing agent, light, heating temperature and diffrent storage condition. Added oxidator (H202 1%) made absorbance get lower 81,69% afler 15 days. The impact of the UV light (25 watt) for 5 days showed that the colour intensity change at the transparent store (72,37%) higher than dark store (65,80%). Color intensity at the diffrent storage condition after 7 days showed that at room temperature (77,17%) higher than refrigator temperature (67, 07%). The heated at 100°C for 120 minute showed the higher change of colour intensity (37,80%).
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