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ASESMEN KOLONISASI JAMUR ENTOMOPATOGEN ASAL SERANGGA DAN TANAH PADA BIBIT JAGUNG DAN PATOGENESITASNYA TERHADAP LARVA Spodoptera frugiperda
Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest of maize that come from America, spreaded into another countries and caused high amount of loss. One of the method to control S. frugiperda was by using entomopathogenic fungi. Entomopathogenic fungi are biological controlling agent to decrease pest's populations by causing the disease in insects through direct contact. The purpose of this study was to determine which entomopathogenic fungi from insects and soil were endophytic in the tested maize plants, To determine the effect of entomopathogenic fungi which were proven to be endophytic on maize growth and to determine the level of pathogenicity to the mortality of S. frugiperda larvae. In this study, the isolates used were isolates previously obtained from infected insects and plant root rhizosphere from South Sumatra. This study used 11 isolates with name, LtTpOI, TaTsOI, TaAlPA, TaSkPA, TaBrPGA, TaCjPGA, LtApPGA, LtKrLH, TaTtLH, TaLmMe, TaPsBA and added control with 3 replications and designed using a completely randomized design. The methods used included testing of endophytic fungi inoculation on maize seedlings and pathogenicity testing of S. frugiperda larvae. The variables observed in this study consisted of conidia density and conidia viability, leaf area eaten, larval body weight, excrement weight, larvae mortality, LT50 and LT95 larvae, percentage of pupae appearing, normal pupae, abnormal pupae, pupa weight, pupa length, Number of adults, normal and abnormal adults, body length of adults, the wingspan of adults, length of life of male and female adults, number of eggs laid and larvae released, germination, length of a radicle, plumule, wet weight and dry weight of maize seed, seedling height, length leaves, leaf width, root length, and number of leaves of corn seeds. The results obtained from 11 isolates of Beauveria sp. which was inoculated into the seeds was that 6 isolates contained endophytic entomopathogenic fungi, namely LtTpOI, TaTsOI, TaAlPA, TaBrPGA, LtApPGA, TaTtLH isolates. The highest percentage of endophytic fungal colonization on corn seedlings was found in TaTtLH isolates which reached (63.33%). The highest mortality (29.33%) was found in TaTtLH isolates with LT50 of 24.46 days and an LT95 of 58.60 days. The lowest mortality was found in TaLmME isolates (2.67%) with LT50 of 44.36 days and LT95 of 76.58 days. Corn seeds that was given endophytic entomopathogenic fungi on average had better growth than the controls and other isolates that were not endophytic. On average corn seedlings treated with endophytic fungi had better growth than the control and other isolates that were not endophytic. The highest seedling growth in all agronomic variables was found in TaTtLH isolates and the lowest was found in TaLmME isolates. The conclucsion of this study was that from 11 entomopathogenic fungi derived by insects and soil, there were 6 endophytic fungi, LtTpOI, TaTsOI, TaAlPA, TaBrPGA, LtApPGA, and TaTtLH. The highest endophytic colonization was found in TaTtLH isolates. The pathogenicity of proven to be endophytic on larva's mortality with different The most pathogenic endophytic fung entomopathogenic fungi levels i were found in TaTtLH isolate.
Inventory Code | Barcode | Call Number | Location | Status |
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2107003751 | T62671 | T626712021 | Central Library (Referens) | Available but not for loan - Not for Loan |
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