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ISOLASI DUA SENYAWA ANTIBAKTERI DARI JAMUR ENDOFITIK Aspergillus sp1 BATANG BROTOWALI (Tinospora crispa L)

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Two compounds of secondary metabolites has been isolated fforn endophytic fungi of brotowali (Tinospora crispa L) stem. Isolation began by cultivation of Aspergillus spi fungi in 4 litres of PDB {Potato Dextrose Broth) media for eight weeks. Medium culture was extracted partially using ethyl acetate and then followed evaporated by evaporation. Separation and purification of isolated compound using gravity column chromatographic technique. Antibacterial activity by disc diffusion method was tested againt E. coli, S. dysentriae, S. aureus, and B.subtilis bacteria. Two isolated compounds one of them is colourless oil (compound 1) while anorther is white crystals (compound 2) with melting point is 155-156°C. Base on spectroscopy analysis data UV, IR, lH-NMR and NMR showed that compound 1 is bis (2-ethylhexyl) phthalate and compound 2 is 5-hydroxy-4 (l-hydroxymethyl)-l-piran-2-on. Antibacterial activity assay showed inhibition zone diameter test againt E. coli, S. dysenteriae, S. aureus, and B. subtilis bacteria compound 1 6.5; 6.7; 6.8; 6.6 mm respectively and compound 2 showed inhibition zone diameter 8.3; 10.0; 12.2; 9.7 mm respectively at concentration 500 ppm.

Availability
Inventory Code Barcode Call Number Location Status
1307001714T65654T656542013Central Library (REFERENCES)Available but not for loan - Not for Loan
Detail Information
Series Title
-
Call Number
T656542013
Publisher
Inderalaya : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sriwijaya.,
Collation
xvii, 55 hlm.; ill.; tab.; 28 cm.
Language
Indonesia
ISBN/ISSN
-
Classification
632.407
Content Type
Text
Media Type
unmediated
Carrier Type
unspecified
Edition
-
Subject(s)
Penyakit jamur pada tanaman pertanian
Jurusan Kimia
Specific Detail Info
-
Statement of Responsibility
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No other version available

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